The outcome of pH over the kinetic constants for that hydrolysis of substrates by NagA was measured within an make an effort to detect People practical groups from the substrate and active web page which might be necessary to be in a specific point out of protonation. The log k
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makes use of a single steel sure exclusively on the β-site. The most Excessive case in point throughout the amidohydrolase superfamily is uronate isomerase from E. coli
-trifluoroacetyl substituted substrate is hydrolyzed 26 times a lot quicker compared to all-natural substrate though the N
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